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Sep 17, 2001 · The Hip1R coiled-coil domain does not contain the clathrin binding motifs L(L,I)(D,E,N)(L,F)(D,E), referred to as the “clathrin box,” or the shorter DLL motif found in many proteins that interact with the TD of clathrin HC (Dell'Angelica et al., 1998; Morgan et al., 2000; ter Haar et al., 2000). Consistent with these observations, we did ... Add the 100 μl Lipofectamine 2000/DNA solution to the plated cells and incubate for ~ 6 h. Wash cells with two volumes of dPBS and add fresh complete RPMI media. 3. It is critical to wash cells with one volume of dPBS and change media every day. 4. Two days posttransfection, cells are now ready for further treatment or confocal microscopy viewing.

Lentivirus was conducted by triple transfection of PANC-1 cells with 4 µ g of miR-451/miR-NC and 4 µ g of lentiviral vector using lipid transfection (Lipofectamine-2000/Plus Reagent, Invitrogen). Then the lentivirus was subcutaneously injected in the flank of the nude mice, respectively.
LipoGene 2000 Star Transfection Reagent Proteinfectin Transfection Reagent DNAfectin 2100 Transfection Reagent NA Lipofectamine 3000 Reagent LipoGene 2000 PLus ...
Lipofectamine® 2000 Reagent Protocol 2013-2-Lipofectamine® 2000 DNA Transfection Reagent Protocol Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations.
Flp-In™ T-REx™-293 cells (Invitrogen), which are commercially available human embryonic kidney derived cells, were cultured in standard DMEM medium supplemented with 200 μM uridine and 2 mM pyruvate and 5% TET free foetal bovine serum (Ozyme) (Spelbrink et al., 2000) plus appropriate antibiotics for transgene selection, and transfected ...
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transfection was conducted with Lipofectamin 2000 plus (ThermoFisherScientific),using200ngofpT81Lucreport-er plasmids, 50 ng of pcDNA3.1-KLF4, 200 ng of pCMVb-galactosidase (Clontech Laboratories, Inc.) to normalize for transfection efficiency, and a carrier plasmid to main-tain a total DNA concentration of 800 ng. After a 48-hour
Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 491204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
I have been using lipofectamine 2000 with AAV293 cells and triple transfection to make AAVs for in vivo injection into mouse brain. It was reported that PEI transfection works well and can allow ...
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  • 안녕하세요~~ㅎ GPCR DNA를 클로닝하고 이제 발현을 보려고 하는데요. 처음엔 293T cell을 썼었는데 이게 나오는건지 안나오는건지 너무 약해서요..
  • Lane 1, cells without any treatment; lane 2, Lipofectamine 2000 plus siRNA; lane 3, free siRNA; lanes 4 and 5, PEG-HAuNS-siRNA and F-PEG-HAuNS-siRNA luc both plus NIR laser irradiation (50 mW/cm 2 for 60 s); lanes 6 to 8, F-PEG-HAuNS-siRNA plus NIR laser irradiation (50 mW/cm 2) for 60, 30, and 0 s; lane 9, F-PEG-HAuNS-siRNA plus NIR laser ...
  • Lipofectamine® 2000 Reagent Protocol 2013-2-Lipofectamine® 2000 DNA Transfection Reagent Protocol Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations.

Enzyme transduction was performed 6 h after plating using Lipofectamine LTX (Invitrogen), Lipofectamine 3000 (Invitrogen) and Proteofectene (Biontex). Proteins were pre-incubated for 20 minutes at 20 °C in 100 μl Optimem (Gibco) with or without 2.5 μl of the transfection reagents, with 10 μl (for 96-well) or 30 μl (for 48-well) being added ...

Fig. 2C shows that the cells in the quiescent state were transfected with oligonucleotides by incubation for 48 h with Lipofectamine 2000, the ClC-3 protein was decreased by 12.7 ± 1.6%, 26.8 ± 2.8%, 57.8 ± 2.4%, and 58.2 ± 2.3% (data from six different experiments; p < 0.01) in 5 μl/ml Lipofectamine 2000 plus 25, 50, 100, and 200 μg/ml ...
As one example, delivery can be via a liposome, using commercially available liposome preparations such as LIPOFECTIN, LIPOFECTAMINE (GIBCO-BRL, Inc., Gaithersburg, Md.), SUPERFECT (Qiagen, Inc. Hilden, Germany) and TRANSFECTAM (Promega Biotec, Inc., Madison, Wis.), as well as other liposomes developed according to procedures standard in the art. Although various optimizations have been compared by combining different chemicals, such as ExGen500 (Fermentas) (Eiges et al., 2001; Matin et al., 2004), calcium phosphate (Darr et al., 2006), FuGENE (Boehringer Mannheim) (Liu et al., 2004), LipofectAMINE Plus (Life Technologies) (Vallier et al. 2004), or Lipofectamine 2000 (Invitrogen) (Hay ...

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Sep 17, 2001 · The Hip1R coiled-coil domain does not contain the clathrin binding motifs L(L,I)(D,E,N)(L,F)(D,E), referred to as the “clathrin box,” or the shorter DLL motif found in many proteins that interact with the TD of clathrin HC (Dell'Angelica et al., 1998; Morgan et al., 2000; ter Haar et al., 2000). Consistent with these observations, we did ...